The Agilent 1290 Infinity II Manual provides comprehensive guidance for operating, installing, and maintaining the system. It covers advanced features, troubleshooting, and essential safety protocols for optimal performance.
1.1 Overview of the Agilent 1290 Infinity II System
The Agilent 1290 Infinity II is a high-performance UHPLC system designed for advanced chromatographic applications. It offers ultra-high pressure, rapid separation, and precise flow control, ensuring exceptional accuracy and efficiency. Compatible with various columns and detectors, it supports diverse analytical needs in research and industrial settings, delivering reliable results across multiple workflows.
1.2 Importance of the User Manual
The user manual is essential for understanding the Agilent 1290 Infinity II system’s operation, installation, and maintenance. It provides detailed instructions for optimizing performance, troubleshooting issues, and ensuring safety. The manual is a critical resource for users to unlock the system’s full potential and comply with operational guidelines effectively.
Key Features of the Agilent 1290 Infinity II
The Agilent 1290 Infinity II features high-pressure pumps, advanced detectors, and compatibility with various chromatography columns. It offers superior performance, precision, and flexibility for demanding analytical applications in modern laboratories.
2.1 High-Pressure Pump Capabilities
The Agilent 1290 Infinity II features a high-pressure pump capable of delivering up to 1300 bar, enabling efficient separation and analysis. Its advanced design supports high flow rates and compatibility with various chromatography columns, including 1.8 µm particles, ideal for UHPLC, reducing runtime and enhancing performance.
2.2 Advanced Detectors and Their Configurations
The Agilent 1290 Infinity II offers advanced detectors, including the Evaporative Light Scattering Detector (ELSD), Diode Array Detector (DAD), and Fluorescence Detector (FLD). These detectors provide high sensitivity and flexibility for various analytes. The DAD features a deuterium lamp for UV detection, while ELSD excels for non-chromophoric compounds, and FLD optimizes fluorescent molecule analysis.
2.3 Compatibility with Various Chromatography Columns
The Agilent 1290 Infinity II supports a wide range of chromatography columns, including 1.8 µm particle size columns for high-resolution separations. It is compatible with various column types, such as reverse-phase, normal-phase, and HILIC, enabling flexibility for diverse analytical applications and ensuring optimal chromatographic performance across different workflows.
System Components and Accessories
The Agilent 1290 Infinity II system comprises key components such as the Flexible Pump Module, Multisampler, and Inline Filters, ensuring high-performance chromatography and system protection;
3.1 Flexible Pump Module
The Flexible Pump Module delivers high-pressure capabilities up to 1300 bar and flow rates up to 5 mL/min, ensuring precise solvent delivery. Its advanced design supports a wide range of chromatographic methods, from analytical to preparative scales, with exceptional accuracy and reliability for optimal performance.
3.2 Multisampler and Its Capabilities
The Multisampler efficiently handles both vials and microtiter plates, supporting up to 1300 bar system pressure. It ensures precise sample injection and optimal chromatographic performance, making it ideal for high-throughput and versatile laboratory workflows. Its design enhances productivity while maintaining accuracy in various analytical applications.
3.4 Inline Filters and Their Role
Inline filters protect the UHPLC column from particulate matter, ensuring optimal system performance. Made from durable materials like stainless steel or PEEK, they effectively prevent clogging. Regular replacement is crucial for maintaining chromatographic efficiency and extending column lifespan, minimizing downtime in high-throughput workflows.
Installation and Setup Guidelines
Proper installation involves unpacking, inventorying components, and connecting modules. Initial calibration ensures system accuracy and prepares the instrument for operation, following detailed manual instructions.
4.1 Unpacking and Inventory of Components
Carefully unpack the Agilent 1290 Infinity II system, ensuring all components are accounted for. Verify the integrity of each part, checking for damage. Organize components systematically, referencing the manual for identification. This step ensures a smooth installation process and prevents missing or damaged items.
4.2 Connecting System Modules
Connect system modules carefully, aligning ports and securing connections firmly. Use the provided adapters for compatibility. Ensure all tubes and fittings are tightly sealed. Refer to the manual for torque specifications. After connecting, perform a leak test to verify system integrity before proceeding with calibration.
4.3 Initial System Calibration
Calibrate the system by priming the pump, checking detector settings, and verifying pressure and flow rate accuracy. Use the holmium oxide filter for wavelength verification. Follow the manual’s step-by-step guide to ensure precise instrument setup and optimal performance before starting analysis.
Operating the Agilent 1290 Infinity II
Operate the system by initializing the pump, setting up chromatographic methods, and running samples. Ensure proper parameter configuration and monitor performance metrics for precise and reliable analysis.
5.1 Basic Operation Steps
Power on the system and initialize the pump. Set method parameters, including flow rate and pressure. Load samples into the autosampler. Start the chromatographic run and monitor real-time data. Ensure all components function within specified limits for precise and accurate results.
5.2 Setting Up a Chromatographic Method
Access the system software to create and configure a chromatographic method. Define parameters like mobile phase composition, flow rate, and gradient. Set injection volume and sample tray configuration. Configure detector settings based on type (DAD, ELSD, FLD) and save the method. Ensure proper communication between software and hardware, and verify with a test run before sample analysis.
5.3 Monitoring System Performance
Monitor real-time system performance through the software interface, tracking pressure, flow rate, and detector signals. Use built-in diagnostics to identify potential issues like leaks or worn parts. Regularly check chromatograms for consistency and reproducibility. Address any anomalies promptly to ensure optimal operation and prevent downtime, maintaining high reliability and accuracy in your analyses.
Maintenance and Troubleshooting
Regular maintenance ensures optimal performance. Clean or replace worn parts, check for leaks, and address error messages promptly. Troubleshooting guides help resolve common issues efficiently.
6.1 Regular Maintenance Procedures
Regular maintenance involves replacing inline filters, cleaning components, and checking seals for wear. Perform leak tests and calibrate detectors as specified. Follow recommended schedules to ensure system longevity and prevent downtime. Use genuine Agilent parts for replacements to maintain performance and warranty compliance.
6.2 Common Issues and Solutions
Common issues include high-pressure leaks, detector malfunctions, and system connection errors. Solutions involve checking O-rings, recalibrating detectors, and verifying tubing connections. Regular maintenance and using genuine Agilent parts help prevent these issues. Refer to the troubleshooting guide for detailed diagnostic steps and repairs to ensure optimal system performance.
6.3 Leak Testing and System Sealing
Regular leak testing ensures system integrity and performance. Inspect connections, O-rings, and sealing components. Address leaks promptly to prevent fluid loss and damage. Use Agilent-approved tools and follow manual guidelines for sealing. Proper sealing maintains high pressure and precision, ensuring reliable chromatographic results and extending system lifespan.
Advanced Features and Customization
The Agilent 1290 Infinity II offers advanced customization options, including the Flexible Cube Module for multi-dimensional chromatography and seamless integration with MS systems for enhanced analytical capabilities.
7.1 Using the Flexible Cube Module
The Flexible Cube Module in the Agilent 1290 Infinity II enables multi-dimensional chromatography by allowing the use of multiple detectors and columns. It enhances separation efficiency and workflow flexibility, accommodating various analytical needs with its modular design and high-pressure capability up to 1300 bar.
7.2 Integrating with MS Systems
The Agilent 1290 Infinity II seamlessly integrates with MS systems, enhancing analytical sensitivity and workflow efficiency. Modules like the MS Flow Modulator (G7170B) optimize coupling, ensuring precise solvent delivery and compatibility with various mass spectrometers for advanced chromatographic and mass analysis applications.
7.3 Optimizing Chromatographic Performance
The Agilent 1290 Infinity II offers advanced features to enhance chromatographic performance. High-pressure capabilities and precise flow rates enable sharp peak resolution. Additionally, compatibility with columns of varying particle sizes, including 1.8 μm, allows for rapid separations and improved sensitivity, ensuring optimal results in diverse analytical workflows.
Safety Precautions and Compliance
Agilent 1290 Infinity II requires careful handling of high-pressure components and use of protective gear. Adherence to regulatory standards and proper waste disposal ensures safe and compliant operation.
8.1 Handling High-Pressure Components
Handling high-pressure components on the Agilent 1290 Infinity II requires extreme caution. Always wear protective gear, avoid direct contact with pressurized parts, and ensure proper inspection of connections. Follow manufacturer guidelines to prevent accidents and maintain system integrity. Never exceed the maximum pressure rating of 1300 bar. Proper handling ensures safe and reliable operation of the system.
8.2 Compliance with Regulatory Standards
The Agilent 1290 Infinity II adheres to international regulatory standards, ensuring compliance with safety and performance requirements. It meets FDA, ISO, and GMP guidelines, making it suitable for global use. Always verify local regulations and follow manufacturer guidelines to maintain compliance during operation and maintenance.
8.3 Proper Disposal of Waste Materials
Proper disposal of waste materials from the Agilent 1290 Infinity II is crucial. Always follow local regulations and guidelines for handling hazardous waste. Recycle materials when possible and ensure safe disposal to minimize environmental impact. Check the manual for specific disposal instructions, especially for contaminated items.
Detectors and Their Configuration
The Agilent 1290 Infinity II features advanced detectors, including ELSD, DAD, and FLD, each offering unique capabilities for precise and versatile chromatographic analysis.
9.1 Evaporative Light Scattering Detector (ELSD)
The Evaporative Light Scattering Detector (ELSD) is a universal detector for non-chromophoric compounds. It operates by nebulizing the eluent, evaporating the solvent, and measuring scattered light. This detector is ideal for high-pressure systems, offering sensitivity and compatibility with a wide range of chromatographic methods.
9.2 Diode Array Detector (DAD)
The Diode Array Detector (DAD) offers high sensitivity and versatility for UV-Vis detection. It features a deuterium lamp for broad wavelength coverage and rapid scanning capabilities. Ideal for chromatographic analysis, the DAD provides accurate spectral data, enabling compound identification and quantification with precision.
9.3 Fluorescence Detector (FLD)
The Fluorescence Detector (FLD) excels in detecting fluorescent compounds with high sensitivity. It is ideal for analyzing samples with natural fluorescence or those requiring derivatization. The FLD offers superior selectivity and is widely used for trace analysis, making it a versatile tool for advanced chromatographic applications.
Accessories and Consumables
The Agilent 1290 Infinity II system requires specific accessories like columns, guard columns, and inline filters to ensure optimal performance and longevity. Spare parts and replacement kits are also available for maintenance and upgrades.
10.1 Columns and Guard Columns
The Agilent 1290 Infinity II supports a wide range of chromatography columns, including those filled with 1.8 µm particles for rapid separation. Guard columns are essential for protecting the main column from contaminants, ensuring extended lifespan and consistent performance. They are specifically designed to withstand high pressure and maintain chromatographic efficiency.
10.2 Inline Filters and Fittings
Inline filters are essential for protecting the Agilent 1290 Infinity II system from particulate matter in samples. Properly fitted, they prevent column clogging and maintain optimal chromatographic performance. Regular inspection and replacement of these components ensure extended system lifespan and reliability;
10.3 Spare Parts and Replacement Kits
Spare parts and replacement kits for the Agilent 1290 Infinity II are designed to maintain system performance and longevity. Genuine Agilent components ensure compatibility and reliability, while replacement kits provide essential items for routine maintenance, preventing downtime and ensuring optimal chromatographic results. Always use authorized parts for system integrity.
Resources and References
The Agilent 1290 Infinity II manual provides access to official Agilent resources, including downloadable PDFs, technical guides, and customer support contacts for troubleshooting and maintenance assistance.
11.1 Downloading the User Manual
The Agilent 1290 Infinity II manual is available for download as a PDF from official Agilent resources or platforms like ManualsLib; Ensure to access the correct version (e.g., G7102A) for accurate system operation and maintenance guidance.
11.2 Agilent Technologies Support
Agilent Technologies offers comprehensive support for the 1290 Infinity II system, including troubleshooting, maintenance, and training. Users can access technical assistance, online resources, and detailed documentation through Agilent’s official website or contact their support team for personalized help.
11.3 Additional Technical Documentation
Additional technical documentation for the Agilent 1290 Infinity II includes user manuals, quick reference guides, and technical notes. These resources cover installation, operation, and troubleshooting, ensuring comprehensive support. They are available online, providing detailed insights into system features, detectors, and advanced customization options.
Warranty and Legal Information
The Agilent 1290 Infinity II is covered by a limited warranty, with terms and conditions outlined in the manual. Legal disclaimers and liability limitations are also detailed.
12.1 Warranty Terms and Conditions
The Agilent 1290 Infinity II is covered under a limited warranty, as detailed in Document No: SD-29000230 Rev. G, effective 10/2024. This warranty outlines specific terms, conditions, and exclusions. Agilent disclaims all other warranties, express or implied. The manual’s contents are proprietary and protected under copyright laws.
12.2 Disclaimer and Limitation of Liability
Agilent disclaims all warranties, express or implied, regarding the 1290 Infinity II manual and its content. The company is not liable for damages arising from improper use, unauthorized modifications, or reliance on the information provided in this document. Liability is strictly limited to terms outlined in the warranty agreement.
12.3 Software License Agreement
The Agilent 1290 Infinity II manual software is protected by Agilent Technologies’ standard commercial license terms. Use, duplication, or disclosure of the software is subject to these terms. Unauthorized modifications or reproductions are prohibited. The software is protected by copyright laws, and all rights are reserved by Agilent Technologies.
The Agilent 1290 Infinity II manual ensures optimal system performance and compliance. By following its guidelines, users can maximize efficiency, safety, and chromatographic results, investing in cutting-edge technology for future analytical needs.
13.1 Summary of Key Points
The Agilent 1290 Infinity II manual highlights advanced features like high-pressure pumps, versatile detectors, and compatibility with various columns. It emphasizes proper installation, operation, and maintenance for optimal performance. Adhering to safety protocols and utilizing resources ensures efficient chromatographic analysis and compliance with regulatory standards, maximizing system longevity and analytical results.
13.2 Final Tips for Effective Use
Regular maintenance ensures optimal performance. Proper calibration enhances accuracy. Utilize the flexible cube module for advanced customization. Adhere to safety guidelines for secure operation. Leverage Agilent’s support resources for troubleshooting. Follow manual instructions carefully to maximize system potential and longevity.